S150-05A
Human Skeletal Muscle Cells: HSkMC: Pre-Screened for Insulin & AMPK signaling, adult
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About This Item
Código UNSPSC:
12352204
Produtos recomendados
fonte biológica
human skeletal muscle (normal limb)
embalagem
pkg of 500,000 cells
modo de crescimento
Adherent
cariótipo
2n = 46
morfologia
Skeletal muscle
técnica(s)
cell culture | mammalian: suitable
doença(s) relevante(s)
muscular dystrophy
Condições de expedição
dry ice
temperatura de armazenamento
−196°C
Descrição geral
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Pre-screened Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle. Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK and Insulin Signaling Pathways.
1. AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise. AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.
2. Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action. Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell.
Pre-screened Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle. Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK and Insulin Signaling Pathways.
1. AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise. AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.
2. Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action. Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell.
Origem de linhagem celular
Muscle
Aplicação
lipid and glucose metabolism, exercise, insulin action, cell signaling
Componentes
Basal Medium containing 10% FBS & 10% DMSO
Nota de preparo
- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Rotina de subcultura
Please refer to the HSkMC Culture Protocol.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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