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Key Documents

R128

Sigma-Aldrich

Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

clone C3-33, ascites fluid

Sinônimo(s):

Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

C3-33, monoclonal

peso molecular

antigen ~565 kDa (non-mammalian vertebrates, a doublet at 565 kDa represents the α and β isoforms)

reatividade de espécies

canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human

concentração

~1 mg/mL

técnica(s)

immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

Descrição geral

RyR1 (ryanodine receptor isoform 1) is a homotetrameric intracellular calcium channel, that is present in skeletal muscle tissue. It is located on human chromosome 19q13.1. In rats, RyR1 and RyR2 are expressed in PASMCs (pulmonary artery smooth muscle cells).

Especificidade

The antibody reacts strongly with RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform), and weakly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform). It reacts with ryanodine receptor in cardiac muscle of canine, rat, finch and pigeon; in visceral smooth muscle of toad; and in rat brain. It reacts with skeletal muscle of fish and the β isoform of frog, but only weakly with skeletal muscle in rabbit and the α isoform of frog. In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in the hippocampus.

Imunogênio

dog cardiac ryanodine receptors (RyR)

Aplicação

Anti-Ryanodine Receptor has been used in immunofluorescence assay.

Ações bioquímicas/fisiológicas

In rats PASMCs (pulmonary artery smooth muscle cells), RyR2 (ryanodine receptor isoform 1) participates in RyR-gated Ca2+ entry. It controls the secretion of insulin and glucose homeostasis. After hemorrhagic shock, RyR2 involves in the progress of vascular bi-phasic reactivity to NE (norepinephrine). In human, RyR1 plays an important role in muscle contraction. RYR1 mutation causes CCD (central core disease) and MHS (malignant hyperthermia susceptibility).

forma física

Containing PBS and 0.05% sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

Internal structure and visualization of transmembrane domains of the RyR1 calcium release channel by cryo-EM
Samso M, et al.
Nature Structural and Molecular Biology, 12(6), 539-544 (2005)
Imaging Ca2+ nanosparks in heart with a new targeted biosensor
Shang W, et al.
Circulation Research, 114(3), 412-420 (2014)
An autosomal dominant congenital myopathy with cores and rods is associated with a neomutation in the RYR1 gene encoding the skeletal muscle ryanodine receptor
Monnier N, et al.
Human Molecular Genetics, 9(18), 2599-2608 (2000)
The pore structure of the closed RyR1 channel
Ludtke SJ, et al.
Structure, 13(8), 1203-1211 (2005)
Glucose-Dependent Insulin Secretion in Pancreatic ?-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors
Llanos P, et al.
PLoS ONE, 10(6) (2015)

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