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Key Documents

P8045

Sigma-Aldrich

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Sinônimo(s):

Biotinylated Protein G, Protein G Biotin, Protein G-Biotin

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

recombinante

expressed in unspecified host

Nível de qualidade

conjugado

biotin conjugate

forma

lyophilized powder

composição

Protein, ~90% biuret

Extensão da rotulagem

2-4 mol d-biotin per mol protein

técnica(s)

direct ELISA: 1:70,000 using human IgG

temperatura de armazenamento

2-8°C

Descrição geral

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

Especificidade

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Aplicação

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.

Embalagem

Package size based on protein content

Outras notas

Truncated - lacks albumin, Fab and membrane binding sites.

Nota de preparo

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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