P7643

PIPES dipotassium salt

≥99% (titration)

• Sinônimo(s): 1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt
• Fórmula empírica (Notação de Hill): C₈H₁₆K₂N₂O₆S₂
• Número CAS: 108321-27-3
• Peso molecular: 378.55
• Número MDL: MFCD00069751
• Código UNSPSC: 12161700
• ID de substância PubChem: 24898738
• NACRES: NA.25

Propriedades

• Nível de qualidade: 200
• Ensaio: ≥99% (titration)
• Formulário: crystalline powder
• faixa de pH útil: 6.1-7.5
• pKa (25 °C): 6.8
• solubilidade: water: 0.25 g/mL, clear, colorless
• aplicação(ões): diagnostic assay manufacturing
• cadeia de caracteres SMILES: [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
• chave InChI: BJQYFCAMUXGZFN-UHFFFAOYSA-L

Descrição

Descrição geral

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Aplicação

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)