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P7457

Sigma-Aldrich

Carboxy-terminal FLAG-BAP™ Fusion Protein

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About This Item

Número MDL:
MFCD00130921
Código UNSPSC:
12352202
NACRES:
NA.32

Formulário

liquid

Nível de qualidade

200

peso molecular

~49 kDa

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

Aplicação

Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).
Learn more product details in our FLAG® application portal.

Ações bioquímicas/fisiológicas

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

Outras notas

Control protein

forma física

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2

Nota de preparo

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Informações legais

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análise (COA)

Lot/Batch Number
SLCB6701
SLBH7385V
SLBF9427V
120M6207
040M6148

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Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
Domanski M, et al.
Biotechniques, 1-1 (2012)
Microbial alkaline phosphatases in bioprocessing
Nalini P, et al.
International Journal of Current Microbiology and Applied Sciences, 4(3), 384-396 (2015)
Maddalena de Virgilio et al.
Journal of experimental botany, 59(10), 2815-2829 (2008-06-10)
Protein bodies (PB) are stable polymers naturally formed by certain seed storage proteins within the endoplasmic reticulum (ER). The human immunodeficiency virus negative factor (Nef) protein, a potential antigen for the development of an anti-viral vaccine, is highly unstable when
Patricia L Pelczar et al.
Journal of bacteriology, 190(16), 5635-5641 (2008-06-17)
GerD of Bacillus subtilis is a protein essential for normal spore germination with either L-alanine or a mixture of L-asparagine, D-glucose, D-fructose, and potassium ions. GerD's amino acid sequence suggests that it may be a lipoprotein, indicating a likely location
Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells
Chubet RG and Brizzard BL
Biotechniques, 20(1), 136-141 (1996)
Exibir todos os documentos relacionados

Artigos

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Conteúdo relacionado

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Expression

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

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