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P6662

Sigma-Aldrich

o-Fenilenediamina

tablet, 1 mg substrate per tablet

Sinônimo(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Fórmula linear:
C6H4(NH2)2·2HCl
Número CAS:
615-28-1
Peso molecular:
181.06
Beilstein:
3912045
Número MDL:
MFCD00012966
Código UNSPSC:
12352204
ID de substância PubChem:
24898787
NACRES:
NA.83

forma

tablet

pf

258 °C (dec.) (lit.)

solubilidade

water: 1 tablet/10 mL, clear, colorless

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

chave InChI

RIIWUGSYXOBDMC-UHFFFAOYSA-N

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

O dicloridrato de o-fenilenodiamina (OPD) é um substrato cromogênico que pode ser usado em procedimentos de ensaio de imunoabsorção enzimática (ELISA), pois utiliza conjugados de peroxidase de rábano. O substrato gera um produto final solúvel de cor laranja-marrom e pode ser lido por espectrofotometria em 450 nm. A reação de OPD pode ser interrompida com HCl 3N ou H2SO4 3 M e lida a 492 nm.

Reconstituição

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Palavra indicadora

Warning

Frases de perigo

H317,H341,H351,H410

Classificações de perigo

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Muta. 2 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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