L7759
Lectin from Arachis hypogaea (peanut)
peroxidase conjugate, lyophilized powder
Sinônimo(s):
PNA, Peanut agglutinin
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.54
Produtos recomendados
fonte biológica
Arachis hypogaea (peanut)
Nível de qualidade
conjugado
peroxidase conjugate
Formulário
lyophilized powder
peroxidase activity
≥20 units/mg protein
técnica(s)
western blot: suitable
temperatura de armazenamento
−20°C
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Aplicação
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Ações bioquímicas/fisiológicas
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Definição da unidade
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
forma física
Contains sodium citrate
Nota de preparo
Prepared from peroxidase (P8375) using a modification of a published method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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