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Key Documents

P9029

Sigma-Aldrich

o-Phenylenediamine

peroxidase substrate, chromogenic, ≥98.0%, powder

Sinônimo(s):

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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About This Item

Fórmula empírica (Notação de Hill):
C6H8N2
Número CAS:
Peso molecular:
108.14
Beilstein:
606074
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:
NACRES:
NA.83

product name

o-Phenylenediamine, Peroxidase substrate, ≥98.0%, powder

densidade de vapor

3.7 (vs air)

Nível de qualidade

pressão de vapor

0.01 mmHg ( 25 °C)

Ensaio

≥98.0%

forma

powder

cor

white to off-white

pb

256-258 °C

pf

98-102 °C

solubilidade

ethanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescência

λex 337 nm; λem 417 nm (Pyruvic acid derivative)

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

chave InChI

GEYOCULIXLDCMW-UHFFFAOYSA-N

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Descrição geral

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Aplicação

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Atenção

May darken in storage.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

276.8 °F - closed cup

Ponto de fulgor (°C)

136 °C - closed cup


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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