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P4399

Sigma-Aldrich

β-Lactamase from Enterobacter cloacae

Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)

Sinônimo(s):

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

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About This Item

Número CAS:
9073-60-3
Número da licença da enzima:
3.5.2.6 (BRENDA, IUBMB)
Número MDL:
MFCD00131819
Código UNSPSC:
12352204
NACRES:
NA.54

tipo

Type III

forma

lyophilized powder

atividade específica

6-18 units/mg protein (using benzylpenicillin)

composição

Protein, ~10%

temperatura de armazenamento

2-8°C

Procurando produtos similares? Visita Guia de comparação de produtos

Aplicação

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4399 is produced from Enterobacter cloacae.

Ações bioquímicas/fisiológicas

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Definição da unidade

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

forma física

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts

Nota de preparo

Chromatographically purified

Nota de análise

Protein determined by biuret

inibidor

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H317,H334

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

76427

Penicillin Amidase from Escherichia coli

P J Johnsen et al.
Genetics, 181(4), 1521-1533 (2009-02-05)
We present a new hypothesis for the selective pressures responsible for maintaining natural competence and transformation. Our hypothesis is based in part on the observation that in Bacillus subtilis, where transformation is widespread, competence is associated with periods of nongrowth
Arnold Louie et al.
Antimicrobial agents and chemotherapy, 56(1), 258-270 (2011-10-26)
New broad-spectrum β-lactamases such as KPC enzymes and CTX-M-15 enzymes threaten to markedly reduce the utility of our armamentarium of β-lactam agents, even our most potent drugs, such as carbapenems. NXL104 is a broad-spectrum non-β-lactam β-lactamase inhibitor. In this evaluation
Ryan M Phelan et al.
Bioorganic & medicinal chemistry letters, 19(4), 1261-1263 (2009-01-27)
An efficient synthesis of a 5-fluorouracil-cephalosporin prodrug is described for use against colorectal and other cancers in antibody and gene-directed therapies. The compound shows stability in aqueous media until specifically activated by beta-lactamase (betaL). The kinetic parameters of the 5-fluorouracil-cephalosporin
Elena De Vecchi et al.
Journal of medical microbiology, 62(Pt 6), 859-863 (2013-03-12)
Urinary tract infections (UTIs) are a common cause of bacteraemia in the elderly and are associated with a high probability of hospitalization. Despite the impact of UTIs on health status and quality of life, a limited number of studies have
A A Alsultan et al.
Journal of medical microbiology, 62(Pt 6), 885-888 (2013-03-23)
Carbapenem-resistant Acinetobacter baumannii is becoming increasingly prevalent in patients with diabetes mellitus in the Middle East. We examined the relationship of these bacteria and their resistance mechanisms to the diabetic disease status of patients in Saudi Arabia. Susceptibilities of 271
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