76427
Penicillin Amidase from Escherichia coli
5-10 units/mg protein
Sinônimo(s):
Penicillin Acylase, Penicillin Amidohydrolase
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About This Item
Produtos recomendados
fonte biológica
Escherichia coli
Nível de qualidade
forma
suspension
atividade específica
5-10 units/mg protein
peso molecular
Mr ~70000
técnica(s)
activity assay: suitable
aplicação(ões)
diagnostic assay manufacturing
temperatura de armazenamento
2-8°C
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Descrição geral
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.
Aplicação
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics. It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp. Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.
Ações bioquímicas/fisiológicas
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression. It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.
Definição da unidade
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
Outras notas
Characterization; In enantioselective resolution; Synthesis of ampicillin and benzylpenicillin
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Penicillin Acylase in the Industrial Production of ?-Lactam Antibiotics
Bruggink A, Roos EC, Vroom ED
Organic Process Research & Development, 2(2), 128-133 (1998)
A. Guy et al.
Bioorganic & Medicinal Chemistry Letters, 3, 1041-1041 (1993)
H J Duggleby et al.
Nature, 373(6511), 264-268 (1995-01-19)
Penicillin acylase (penicillin amidohydrolase, EC 3.5.1.11) is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. It is used on an industrial scale for the production of 6-aminopenicillanic acid, the starting material for the synthesis of semi-synthetic penicillins. Its
Ampicillin and cephalexin synthesis catalysed by E. coli penicillin amidase. Yield increase due to substrate recycling
Kasche, V
Biotechnology Letters, 7, 877-882 (1985)
V Kasche et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 365(12), 1435-1443 (1984-12-01)
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl
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