P2545
Protein A–Agarose
saline suspension
Sinônimo(s):
Protein A resin
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About This Item
Produtos recomendados
fonte biológica
Staphylococcus aureus
Nível de qualidade
Formulário
saline suspension
Extensão da rotulagem
~2 mg per mL
ativação da matriz
cyanogen bromide
ligação da matriz
amino
espaçador de matriz
1 atom
capacidade
≥15 mg/mL binding capacity (human IgG)
temperatura de armazenamento
2-8°C
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Descrição geral
Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.
Aplicação
Protein A−Agarose has been used in the immunoprecipitation
- of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
- of prolyl hydroxylase domain 2 N27 cells
- of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells
Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Ações bioquímicas/fisiológicas
Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.
forma física
Suspension in 0.5 M NaCl containing preservative
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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D J McCool et al.
The Biochemical journal, 312 ( Pt 1), 125-133 (1995-11-15)
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete MUC2 without the addition of secretagogues. Treatment of the LS180 cells with
PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
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D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins
McCaw TR, et al.
Biotechnology Progress, 30(5), 1125-1136 (2014)
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