MAK171

Colorimetric Biotin Assay Kit

sufficient for 200 colorimetric assays

• Sinônimo(s): Biotin Quantitation Kit
• Número CE: 200-399-3
• Código UNSPSC: 12352203
• NACRES: NA.84

Propriedades

• método de detecção: colorimetric
• doença(s) relevante(s): dermatological diseases; neurological disorders
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Biotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.

Adequação

Suitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution

Princípio

This Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.

Nota de preparo

Storage and Stability: The kit is shipped on dry ice and storage at –20 °C, protected from light, is recommended.

Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids