BK101
ImmunoProbe™ Biotinylation Kit
Sinônimo(s):
Biotinylation Kits
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About This Item
Código UNSPSC:
41116134
eCl@ss:
34058011
NACRES:
NA.32
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temperatura de armazenamento
2-8°C
Nível de qualidade
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Descrição geral
For custom biotinylation of proteins, Sigma offers kits for conjugation on two different scales. The biotin-avidin system has become popular when high sensitivity and specificity are desired. Biotin can be conjugated to antibodies, lectins, enzymes, and other proteins. The protocols in the kits have been optimized for antibodies. Avidin binds to biotin with a high affinity (Ka = 1015 M) and specificity. When conjugated to enzymes or fluorochromes avidin provides a means of identifying biotinylated compounds via enzymatic conversion of substrate to form a visible product or detection of fluorescence by spectrophotometry or flow cytometry.
Aplicação
Biotin has been modified with aminocaproate, then activated via an ester linkage with sulfo-N-hydroxysuccinimide (BAC-Sulfo-NHS). Aminocaproate provides a six-carbon spacer that reduces steric hindrance on the biotin and improves accessibility to the binding site on avidin. Sulfonation of the hydroxysuccinimide increases the polarity of the reagent, allowing it to dissolve easily in aqueous buffer. The ester provides a carbonyl carbon adjacent to a labile ester linkage as a target for primary amine side chains of accessible lysine residues, joining the biotinamidocaproate to the protein via an amide bond.
ImmunoProbe™ Biotinylation Kit has been used for preparation of biotin labeled antibodies.
Características e benefícios
- Complete protocols for labeling and assay
- BAC-Sulfo-NHS is completely water soluble
- No DMF or DMSO needed
- Biotinylation occurs near neutral pH and physiological ionic strength, avoiding harsh conditions that could damage sensitive proteins
- Fast separation of conjugate from reactants using gel filtration
- 2-5 molar ratio biotin to protein in conjugate (for antibodies)
1 mg protein per reaction (B-TAG)
10 mg protein per reaction (BK-101)
• Sufficient reagents for at least 5 labelings
Embalagem
Kit includes all reagents necessary for biotinylation and for determination of biotin/protein molar ratio.
Nota de análise
Procedure:
Conjugation is performed in four easy steps:
1. Reconstitute BAC-Sulfo-NHS with Phosphate Buffer (PB).
2. Add BAC-Sulfo-NHS to protein and allow to react for 30 minutes at room temperature.
3. Separate the conjugate from the reactants on gel filtration.
4. Assay the conjugate for biotin incorporation by the avidin-HABA assay (BK-101 only). Conjugate is ready to use.
Conjugation is performed in four easy steps:
1. Reconstitute BAC-Sulfo-NHS with Phosphate Buffer (PB).
2. Add BAC-Sulfo-NHS to protein and allow to react for 30 minutes at room temperature.
3. Separate the conjugate from the reactants on gel filtration.
4. Assay the conjugate for biotin incorporation by the avidin-HABA assay (BK-101 only). Conjugate is ready to use.
Informações legais
ImmunoProbe is a trademark of Sigma-Aldrich Co. LLC
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
10 - Combustible liquids
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