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MAK022

Sigma-Aldrich

Calcium Colorimetric Assay

sufficient for 250 colorimetric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 250 colorimetric tests

aplicação(ões)

cosmetics
food and beverages

método de detecção

colorimetric

doença(s) relevante(s)

neurological disorders; hematological disorder

temperatura de armazenamento

2-8°C

Descrição geral

Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.

Aplicação

Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Princípio

In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg.

substituído por

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Descrição
Preços

Pictogramas

Corrosion

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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