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M2003

Sigma-Aldrich

MTT Formazan

powder

Sinônimo(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

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About This Item

Fórmula empírica (Notação de Hill):
C18H17N5S
Número CAS:
57360-69-7
Peso molecular:
335.43
Número MDL:
MFCD00080731
Código UNSPSC:
12171500
ID de substância PubChem:
24896591
NACRES:
NA.47

Ensaio

≥97% (TLC)

Nível de qualidade

200

forma

powder

cor

purple

solubilidade

acetone: 1 mg/mL

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

chave InChI

GIFKVQOPAJDJLO-SZVLODPRSA-N

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Aplicação

MTT Formazan has been used to measure cell viability.

Ações bioquímicas/fisiológicas

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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