X4626

XTT sodium salt

powder, BioReagent, suitable for cell culture

• Sinônimo(s): 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• Fórmula empírica (Notação de Hill): C₂₂H₁₆N₇NaO₁₃S₂
• Número CAS: 111072-31-2
• Peso molecular: 673.52
• Número MDL: MFCD00083517
• Código UNSPSC: 12352207
• ID de substância PubChem: 24902150

Propriedades

• linha de produto: BioReagent
• Nível de qualidade: 200
• Ensaio: ≥90%
• forma: powder
• condição de armazenamento: dry at room temperature
• técnica(s): cell culture | mammalian: suitable; protein quantification: suitable
• aplicação(ões): detection
• método de detecção: colorimetric
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1
• chave InChI: JACYMBNQPPWQML-UHFFFAOYSA-M

Descrição

Descrição geral

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Aplicação

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Ações bioquímicas/fisiológicas

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Princípio

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Informações de segurança

• Código de classe de armazenamento: 4.1A - Other explosive hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)