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Merck
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Key Documents

L7914

Sigma-Aldrich

Lipoprotein, low density from human plasma

≥95% (SDS-PAGE), solution

Sinônimo(s):

β-Lipoprotein, LDL, Low density lipoprotein

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About This Item

Número CAS:
Número MDL:
Código UNSPSC:
12352211
NACRES:
NA.25

fonte biológica

human plasma

Nível de qualidade

Ensaio

≥95% (SDS-PAGE)

forma

solution

nº de adesão UniProt

grupo funcional

ester
phospholipid

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Informações sobre genes

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Aplicação

Lipoprotein, low density from human plasma has been used:
  • as an additive in cholesterol-free RPMI media
  • as a component in buffer to perform an assay for measuring lipid peroxidation
  • to stimulate hepatic macrophages
  • as a plasma protein to determine pazopanib unbound fraction (fu%) by equilibrium dialysis

Ações bioquímicas/fisiológicas

LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake.

Atenção

Freezing of lipoprotein solutions may cause structural or compositional changes.

propriedades físicas

Low density lipoproteins are smaller than VLDL and IDL (26 nm) (MW approximately 3.5 million) and more dense (~1.04). The protein component of LDL is apolipoprotein B100. LDL contains 20-22% protein, 10-15% triglycerides, 20-28% phospholipids, 37-48% cholesteryl esters and 8-10% cholesterol.

forma física

Solution in 150 mM NaCl and 0.01% EDTA, pH 7.4

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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General Leung et al.
Radiology, 257(2), 470-476 (2010-09-11)
To correlate the effect of red blood cell hemoglobin on signal generation during magnetic resonance (MR) imaging and local oxidation of low-density lipoprotein (LDL). Informed consent was obtained from all volunteers participating in this study, which was approved by the
Isolation and analysis of human plasma lipoproteins accumulating postrandial in an intermediate density fraction (d 1.006--1.019 g-ml).
R Fellin et al.
Clinica chimica acta; international journal of clinical chemistry, 54(3), 325-333 (1974-08-20)
Shu-Ping Hui et al.
Analytical and bioanalytical chemistry, 403(7), 1831-1840 (2012-03-01)
1-Palmitoyl-2-linoleoylphosphatidylcholine monohydroperoxide (PC 16:0/18:2-OOH) and 1-stearoyl-2-linoleoylphosphatidylcholine monohydroperoxide (PC 18:0/18:2-OOH) were measured by liquid chromatography/mass spectrometry (LC/MS) using nonendogenous 1-palmitoyl-2-heptadecenoylphosphatidylcholine monohydroperoxide as an internal standard. The calibration curves for synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, which were obtained by direct injection
Ezetimibe suppresses development of liver tumors by inhibiting angiogenesis in mice fed a high-fat diet
Miura K, et al.
Cancer Science, 110(2), 771-771 (2019)
Matthew R Sobansky et al.
Analytical and bioanalytical chemistry, 403(2), 563-571 (2012-02-23)
Columns containing immobilized low-density lipoprotein (LDL) were prepared for the analysis of drug interactions with this agent by high-performance affinity chromatography (HPAC). R/S-Propranolol was used as a model drug for this study. The LDL columns gave reproducible binding to propranolol

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