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Documentos Principais

L6136

Sigma-Aldrich

Lipopolysaccharides from Serratia marcescens

purified by phenol extraction

Sinônimo(s):

LPS

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About This Item

Número MDL:
MFCD00131520
Código UNSPSC:
12352201
NACRES:
NA.25

fonte biológica

Serratia marcescens

Nível de qualidade

200

forma

lyophilized powder

purificado por

phenol extraction

Impurezas

<3% Protein (Lowry)

cor

white to yellow cast

solubilidade

water: 4.90-5.10, faintly hazy to hazy, colorless to faint yellow or tan

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This product is phenol extracted from Serratia marcescens. The source strain is ATCC 21639.

Ações bioquímicas/fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparo

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Outras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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H Heremans et al.
American journal of respiratory and critical care medicine, 161(1), 110-117 (2000-01-05)
Mice given lipopolysaccharide (LPS) intravenously developed lung edema, which was maximum after 6 h. Tumor necrosis factor, interleukin 12 (IL-12), IL-6, and interferon-gamma (IFN-gamma) appeared in the serum, and levels of nitrogen oxide (NO) derivatives were increased in serum and
Mollie R Sorrell et al.
Journal of insect physiology, 126, 104097-104097 (2020-08-14)
Fragile X syndrome (FXS), caused by a mutation in the Fragile X Mental Retardation 1 (FMR1) gene, is a common form of inherited mental retardation. Mutation of the gene leads to a loss of the gene product Fragile X Mental Retardation Protein (FMRP). While a loss
M G Zenobi et al.
Journal of dairy science, 103(3), 2200-2216 (2020-01-20)
Objectives were to evaluate the effect of prepartum energy intake and peripartal supplementation of ruminally protected choline (RPC) on select indicators of immune status in blood plasma and on lipopolysaccharide-stimulated blood cells ex vivo. At 47 ± 6 d before
Ching-Hua Hsieh et al.
Journal of biomedical science, 19, 69-69 (2012-08-02)
Lipopolysaccharide (LPS) is recognized as the most potent microbial mediator presaging the threat of invasion of Gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. This study was designed to examine the microRNA (miRNA) expression in whole
Victor Meseguer et al.
Nature communications, 5, 3125-3125 (2014-01-22)
Gram-negative bacterial infections are accompanied by inflammation and somatic or visceral pain. These symptoms are generally attributed to sensitization of nociceptors by inflammatory mediators released by immune cells. Nociceptor sensitization during inflammation occurs through activation of the Toll-like receptor 4

Conteúdo relacionado

Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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