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Documentos Principais

L4268

Sigma-Aldrich

Lipopolysaccharides from Klebsiella pneumoniae

purified by phenol extraction

Sinônimo(s):

LPS

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About This Item

Número MDL:
MFCD00131520
Código UNSPSC:
12352201
NACRES:
NA.25

fonte biológica

bacterial (Klebsiella pneumoniae)

Nível de qualidade

200

Formulário

lyophilized powder

purificado por

phenol extraction

Impurezas

<3% Protein (Lowry)

cor

white to faint yellow

solubilidade

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This product is phenol extracted from Klebsiella pneumoniae. The source strain is ATCC 15380.

Ações bioquímicas/fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparo

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Outras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
059M4188V
12181004
068M4069V
116M4057V
065M4026V

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Conteúdo relacionado

Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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