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Documentos Principais

L5505

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

lyophilized powder

Sinônimo(s):

Ulex europaeus agglutinin, UEA-I

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

Formulário

lyophilized powder

Nível de qualidade

potência

<8 μg/mL agglutination activity

composição

Protein, ≥80% biuret

Impurezas

salt, essentially free

temperatura de armazenamento

2-8°C

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Ações bioquímicas/fisiológicas

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Ligação

Conjugates are prepared from affinity purified lectin.

Nota de preparo

Purified by affinity chromatography using the method of Pereira.

Nota de análise

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Immunochemical studies on the combining site of the blood group H-specific lectin 1 from Ulex europeus seeds.
M E Pereira et al.
Archives of biochemistry and biophysics, 185(1), 108-115 (1978-01-15)
D Guy et al.
British journal of cancer, 42(6), 915-921 (1980-12-01)
Present evidence suggests that the cell surface has an important role in metastasis. To examine this idea further, the surface properties of single cells isolated from the primary growths of a liver-metastasizing (ML) and a non-metastasizing (NML) lymphosarcoma were compared
R T Sawyer et al.
Infection and immunity, 60(3), 1041-1046 (1992-03-01)
The isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of Candida albicans. After mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) C. albicans CFU
M Kristensen et al.
Scandinavian journal of medicine & science in sports, 25 Suppl 2, 40-48 (2015-06-18)
We investigated the effect of alpine skiing for 12 weeks on skeletal muscle characteristics and biomarkers of glucose homeostasis and cardiovascular risk factors. Twenty-three patients with a total knee arthroplasty (TKA) were studied 2.9 ± 0.9 years (mean ± SD) after the operation. Fourteen

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