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Documentos Principais

L3019

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), lyophilized powder

Sinônimo(s):

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

Nível de qualidade

forma

lyophilized powder

potência

≤8 μg per mL agglutination activity (using human blood group B erythrocytes)
≥125 μg per mL agglutination activity (using human blood group A erythrocytes)

composição

Protein, ~95% E1%/280

temperatura de armazenamento

−20°C

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Ações bioquímicas/fisiológicas

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Outras notas

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Qualidade

Highly purified.

forma física

Contains sodium citrate

Nota de análise

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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