I1408

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

• Sinônimo(s): Affinity Gel
• Código UNSPSC: 41106500
• NACRES: NA.56

Propriedades

• forma: (1:1 suspension in a 30% ethanol solution)
• prazo de validade: 1 yr
• técnica(s): affinity chromatography: suitable
• Impurezas: ≤0.08 Metal Contamination (μmol/ml packed gel)
• matriz: 6% beaded agarose
• capacidade: 10-30 μmol/mL binding capacity (Cu⁺²)
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Aplicação

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

forma física

1:1 suspension in a 30% ethanol solution

Nota de preparo

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Outras notas

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P210 - P233 - P240 - P241 - P242 - P243
• Classificações de perigo: Flam. Liq. 3
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 120.2 °F - closed cup
• Ponto de fulgor (°C): 49 °C - closed cup
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter