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Key Documents

GE17-6003-19

DeStreak Rehydration Solution

Cytiva 17-6003-19, pkg of 5 × 3 mL

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About This Item

Código UNSPSC:
41105323
NACRES:
NA.25

forma

liquid

Características

wetted part: no

embalagem

pkg of 5 × 3 mL

fabricante/nome comercial

Cytiva 17-6003-19

condição de armazenamento

(Rehydration Solution -20 °C Reagent 2 - 8 °C)

cor

Colorless

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

DeStreak Rehydration Solution.

Aplicação

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.

DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak Reagent is also available separately in a 1-ml pack size.

Características e benefícios

  • Improves reproducibility and quality of 2-D gels by preventing streaking.
  • Eliminates extra spots caused by nonspecific oxidation of Proteins.
  • Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
  • Highly suitable for both analytical and preparative gels.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

DeStreak is a trademark of Cytiva

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Código de classe de armazenamento

12 - Non Combustible Liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

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Artigos

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protocolos

This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.

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