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Documentos Principais

GE17-6003-18

DeStreak Reagent

Cytiva 17-6003-18

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About This Item

Número CE:
Código UNSPSC:
41105323
NACRES:
NA.25

Formulário

liquid

Características

wetted part: no

embalagem

pkg of 1 mL

fabricante/nome comercial

Cytiva 17-6003-18

condição de armazenamento

(Rehydration Solution -20)

cor

Colorless

temperatura de armazenamento

2-8°C

Descrição geral

DeStreak Reagent.

Aplicação

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.

DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak Reagent is also available separately in a 1-ml pack size.

Características e benefícios

  • Improves reproducibility and quality of 2-D gels by preventing streaking.
  • Eliminates extra spots caused by nonspecific oxidation of Proteins.
  • Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
  • Highly suitable for both analytical and preparative gels.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

DeStreak is a trademark of Cytiva

Pictogramas

Skull and crossbones

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 3 Oral - Eye Dam. 1

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

233.6 °F - closed cup

Ponto de fulgor (°C)

112 °C - closed cup


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Certificados de análise (COA)

Lot/Batch Number

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Sigma-Aldrich

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CHAPS hydrate BioReagent, suitable for electrophoresis, ≥98% (HPLC)

Sigma-Aldrich

C9426

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Glicerol for molecular biology, ≥99.0%

Sigma-Aldrich

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Artigos

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protocolos

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from GE Healthcare.

Rehydration Solutions

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

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