GE80-6483-56
2-D Quant Kit
Cytiva 80-6483-56
Sinônimo(s):
2D Quantification Kit, Protein Quant Kit
About This Item
Produtos recomendados
Características
wetted part: no
fabricante/nome comercial
Cytiva 80-6483-56
embalagem
pkg of 500 assays
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Common spectrophotometric methods of quantitating protein rely either on Coomassie dye binding or protein-catalyzed reduction of cupric (Cu2+) ion to cuprous (Cu+) ion. Dye-binding assays cannot be used in the presence of any reagent that also binds the dye. This includes carrier ampholytes and detergents such as PlusOne CHAPS, SDS, and Triton X-100. Assays that depend on the reduction of cupric ion cannot be used in the presence of reductants such as DTT, or in the presence of reagents that form complexes with cupric ion, such as thiourea or EDTA.
The 2-D Quant Kit procedure works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein. Precipitated proteins are resuspended in a copper-containing solution and unbound copper is measured with a colorimetric agent. The absorbance at 480 nm is inversely related to the protein concentration. The assay has a linear response to protein in the range of 0 to 50 μg and recommended sample volume is 1 to 50 μ.
Características e benefícios
- Accurately determine protein concentration in the presence of 2% SDS, 1% DTT, 8 M urea, 2 M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer.
- Quantitatively precipitates proteins while leaving interfering substances behind.
- Linear response in the range of 0 to 50 μg protein, with recommended sample volumes of 1 to 50 μ.
Armazenamento e estabilidade
Nota de análise
Palavra indicadora
Danger
Frases de perigo
Código de classe de armazenamento
8B - Non-combustible corrosive hazardous materials
Certificados de análise (COA)
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Protocolos
2-D Quant Kit from GE Healthcare Lifescience is designed to accurately determine protein concentrations in samples for electrophoresis.
Mini Dialysis Kit from GE Healthcare Lifescience is designed for the dialysis of small sample volumes with minimal handling and sample loss and reduces the pronounced streaking on 2-D gels caused by low-molecular-weight contaminants.
2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.
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