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Key Documents

GE17-0403-03

HiTrap® Protein A High Performance

Cytiva 17-0403-03, pack of 5 × 5 mL

Sinônimo(s):

HiTrap Protein A HP, high performance column, antibody purification

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

native protein A

embalagem

pack of 5 × 5 mL

fabricante/nome comercial

Cytiva 17-0403-03

condição de armazenamento

(20% Ethanol)

Parâmetros

42 psi

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

tamanho

Tamanho médio da partícula

34 μm

cleaning

2-10

working range

3-9

temperatura de armazenamento

2-8°C

Descrição geral

HiTrap® Protein A HP is a prepacked Protein A Sepharose High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient formulation.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características e benefícios

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

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