G115060

DMT-dG(dmf) Phosphoramidite

configured for ABI

• Sinônimo(s): DMT-dG(dmf) Amidite, N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
• Fórmula empírica (Notação de Hill): C₄₃H₅₃N₈O₇P
• Número CAS: 330628-04-1
• Peso molecular: 824.90
• Número MDL: MFCD00797420
• Código UNSPSC: 12352200
• ID de substância PubChem: 329799826
• NACRES: NA.51

Propriedades

• tipo: for DNA synthesis
• Nível de qualidade: 200
• linha de produto: Proligo Reagents
• Ensaio: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• forma: powder
• técnica(s): oligo synthesis: suitable
• cor: white to off-white
• λ: conforms (UV/VIS Identity)
• compatibilidade: configured for ABI
• perfil de nucleosídeo: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• temperatura de armazenamento: -10 to -25°C
• cadeia de caracteres SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
• chave InChI: YRQAXTCBMPFGAN-UJASEYITSA-N

Descrição

Descrição geral

"Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamidine (dmf) baseprotecting group enables the rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.Key Features of dG(dmf) :

• dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for ABI Synthesizers."

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable