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F3046

Sigma-Aldrich

Monoclonal Anti-Actin–FITC antibody produced in mouse

clone AC-40, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):

Monoclonal Anti-Actin

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

200

conjugado

FITC conjugate

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

AC-40, monoclonal

forma

buffered aqueous solution

peso molecular

antigen 42 kDa

reatividade de espécies

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

técnica(s)

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

Isotipo

IgG2a

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ACTA1(58) , ACTA1(58) , ACTA1(58) , ACTA2(59) , ACTA2(59) , ACTA2(59) , ACTB(60) , ACTB(60) , ACTB(60) , ACTC1(70) , ACTC1(70) , ACTC1(70) , ACTG1(71) , ACTG1(71) , ACTG1(71) , ACTG2(72) , ACTG2(72) , ACTG2(72)
mouse ... Acta1(11459) , Acta1(11459) , Acta1(11459) , Acta2(11475) , Acta2(11475) , Acta2(11475) , Actb(11461) , Actb(11461) , Actb(11461) , Actc1(11464) , Actc1(11464) , Actc1(11464) , Actg1(11465) , Actg1(11465) , Actg1(11465) , Actg2(11468) , Actg2(11468) , Actg2(11468)
rat ... Acta1(29437) , Acta1(29437) , Acta1(29437) , Acta2(81633) , Acta2(81633) , Acta2(81633) , Actb(81822) , Actb(81822) , Actb(81822) , Actc1(29275) , Actc1(29275) , Actc1(29275) , Actg1(287876) , Actg1(287876) , Actg1(287876) , Actg2(25365) , Actg2(25365) , Actg2(25365)

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Descrição geral

Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.

Imunogênio

synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone

Aplicação

Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.
Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Ações bioquímicas/fisiológicas

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Outras notas

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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K Sutoh et al.
Biochemistry, 25(20), 6186-6192 (1986-10-07)
An antibody was raised against the N-terminal 18 residues of rabbit skeletal muscle actin. By the use of this antibody as the N-terminal probe of actin and the fluorescent label at Cys-374 as its C-terminal probe, binding sites of depactin
J L Lessard
Cell motility and the cytoskeleton, 10(3), 349-362 (1988-01-01)
Two IgG1, kappa monoclonal antibodies (Mab) against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen. One Mab, designated B4, shows a preferential reactivity toward enteric smooth muscle actin but also cross-reacts
Kimberly A Moore et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 232(2), 268-281 (2004-12-23)
Local alterations in the mechanical compliance of the basement membrane that alter the level of isometric tension in the cell have been postulated to influence tissue morphogenesis. To explore whether cell tension contributes to tissue pattern formation in vivo, we
I M Herman
Current opinion in cell biology, 5(1), 48-55 (1993-02-01)
The actin supergene family encodes a number of structurally related, but perhaps functionally distinct, protein isoforms that regulate contractile potential in muscle tissues and help to control the shape as well as the motility of non-muscle cells. In spite of
The actin gene family: function follows isoform.
Perrin B J and Ervasti J M
Cytoskeleton (Hoboken, N.J.), 67(10), 630-634 (2010)
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