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E7510

Sigma-Aldrich

Earle′s Balanced Salt Solution 10x

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

Earle’s Balanced Salts, EBSS

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

esterilidade

sterile-filtered

Formulário

liquid

concentração

10 ×

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L

Condições de expedição

ambient

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Descrição geral

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Aplicação

Earle′s Balanced Salt Solution 10x has been used:
  • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
  • in the swim-up method to prepare human sperm sample
  • in the steady-state medium to culture bovine primary mammary epithelial cells

Características e benefícios

  • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
  • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
  • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Reconstituição

Supplement with 2.2 g/L sodium bicarbonate at 1×.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Autophagy is a housekeeping lysosomal degradation pathway important for cellular survival, homeostasis and function. Various disease models have shown that upregulation of autophagy may be beneficial to combat disease pathogenesis. However, despite several recently reported small-molecule screens for synthetic autophagy

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