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Sigma-Aldrich

Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

Sinônimo(s):

Cellular glutathione peroxidase, c-GPx

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.32

Nível de qualidade

200

uso

sufficient for 100 tests

prazo de validade

Kit is stable for 24 months when unopened.

método de detecção

colorimetric

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Adequação

Suitable for the measureent of glutathione peroxidase in tissue extracts

Princípio

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Outras notas

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Somente componentes do kit

Nº do produto
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  • tert-Butyl hydroperoxide 1 mL

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Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

5.2 - Organic peroxides and self-reacting hazardous materials

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Certificados de análise (COA)

Lot/Batch Number
078M4136V
11180343
117M4119V
11170527
096M4095V

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V R Arruda et al.
Neoplasma, 43(2), 99-102 (1996-01-01)
Red cell antioxidant enzymes have been recently studied in malignant lymphomas and the results are controversial. Hairy cell leukemia is a rare chronic lymphoproliferative disorder originating probably in a pluripotent stem cell. In the present study, glutathione peroxidase (Gpx), reduced
B L Gupta et al.
Biochemistry and molecular biology international, 46(6), 1145-1152 (1999-01-19)
Experimentally induced diabetic rats were treated separately with insulin and vanadate. The activities of hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6PDH) were increased in reticulocyte hemolysate isolated from the diabetic rats and were restored to normal levels by insulin. The restoration
I Mavelli et al.
European journal of biochemistry, 139(1), 13-18 (1984-02-15)
Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis.
Matthias L Jauslin et al.
Human molecular genetics, 11(24), 3055-3063 (2002-11-06)
Friedreich Ataxia (FRDA), the most prevalent of the inherited ataxias, is a multi-systemic disease with loss of sensory neurons and life-threatening hypertrophic cardiomyopathy as its most severe manifestations. Reduced levels of the mitochondrial protein frataxin lead to cell-damaging oxidative stress
Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.
D E Paglia et al.
The Journal of laboratory and clinical medicine, 70(1), 158-169 (1967-07-01)
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