Eos-HL-60

96100920, human blood, Lymphoblastoid

• Código UNSPSC: 41106514
• fonte biológica: human blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: Eos-HL-60, 96100920
• fonte biológica: human blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian promyelocytic leukaemia

Descrição de linhagem celular

Eos-HL-60 is a variant of the promyelocytic cell line HL60 established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling

Aplicação

Study of production and function of human eosinophils, analysis of differentiation.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

Meio de cultura

IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Outras notas

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