DMS 79

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95062824, human lung, Irregular

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human lung
• embalagem: tube of 5 μg 95062824-DNA-5UG; pkg of vial of cells 95062824-1VL
• modo de crescimento: Aggregates in suspension
• cariótipo: Aneuploid
• morfologia: Irregular
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human lung small cell carcinoma

Descrição de linhagem celular

DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, β-endorphin, 17-β-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatstatin-like imuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice. Cells grow as irregularly shaped aggregates.

Aplicação

Human lung tumour model, carcinogenesis studies.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rotina de subcultura

Maintain cultures between 3 ? 9 x 100,000 cells/ml; 5% CO₂; 37°C. Only dilute 1:2 as a maximum. This cell line grows as clumps in suspension which can be easily dispersed with pipetting. Trypsinisation is not necessary. Routine splitting can therefore

Outras notas

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