VA-ES-BJ

94092303, human skin (bone marrow metastasis), Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: VA-ES-BJ, 94092303
• fonte biológica: human skin (bone marrow metastasis)
• modo de crescimento: Adherent
• cariótipo: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
• morfologia: Epithelial
• produtos: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• receptores: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human skin epithelioid sarcoma

Descrição de linhagem celular

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Aplicação

Study of tumours. Tumourigenic in nude mice.

Meio de cultura

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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