TGP 54

94022430, mouse pancreas, Not specified

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: TGP 54, 94022430
• fonte biológica: mouse pancreas
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Somatostatin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse pancreatic islet cell tumour

Descrição de linhagem celular

TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

Aplicação

Study of exocrine pancreas carcinomas, hormone secretion studies.

Meio de cultura

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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