CRI-G5

88031603, rat pancreas, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: CRI-G5, 88031603
• fonte biológica: rat pancreas
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Insulin and glucagon
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat NEDH islet tumour

Descrição de linhagem celular

Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (Sigma Catalogue number. 88031604).

Aplicação

Hormone studies

Meio de cultura

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. The cells tend to grow in clumps and ribbons.

Outras notas

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