NIH 3T3 Cell Line murine

93061524, mouse embryo, Fibroblast

• Sinônimo(s): 3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: NIH 3T3 Cell Line murine, 93061524
• fonte biológica: mouse embryo
• descrição: Embryonic fibroblast
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Swiss NIH embryo

Descrição de linhagem celular

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

Aplicação

DNA transfection studies

NIH 3T3 Cell Line has been used to:

• study calcium-mediated actin reset (CaAR) in response to physiological changes
• study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
• determine the viral titer of the retroviral construct -ST40hTNFα

Meio de cultura

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

Outras notas

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