M. dunni (Clone III8C)

94101211

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse tail
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Ecotropic Murine leukemia virus (MuLV)
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse tail, normal fibroblast

Descrição de linhagem celular

Derived from the tail tissue of a normal female mouse, Mus dunni. The cells were cloned at passage 14 using Tererasiki plates. M. dunni (Clone III8C) supports the replication of all four classes of murine leukaemia viruses (MuLV); ecotropic, amphotropic, xenotropic and mink cell focus-forming viruses (MCF), showing cytopathic effect. The cells lack most endogenous murine leukaemia virus-related sequences. They are resistant to infection by Moloney MuLV, due to post-translational modifications of the ecotropic MuLV receptor. Confluent cultures will remain more firmly attached in RPMI 1640 or DMEM + 10% FBS during virus assays.

Aplicação

Detection of replication-competent retroviruses in gene therapy.

Meio de cultura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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