SR-4987

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94102704, mouse bone, Fibroblast-like

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse bone
• embalagem: tube of 5 μg 94102704-DNA-5UG; pkg of vial of cells 94102704-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast-like
• produtos: Macrophage colony stimulating factor (M-MCF)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse bone marrow stromal cells

Descrição de linhagem celular

A bone marrow-derived stromal cell line established by A Pessina in 1987 from a normal female 5 month old mouse. A bone marrow culture was treated with the supernatant from Y1 cells which actively produce viral C-particles (MuLV). This fibroblast-like cell line produces macrophage-colony stimulating factor (M-MCF) and exhibits a high sensitivity to fibroblast growth factor (FGF) in clonogenic assays. The cells produce sarcomas in syngenic mice and have a reported doubling time of 14.7 hours.

Meio de cultura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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