EJG

93051803, bovine adrenal gland, Endothelial

• Código UNSPSC: 41106514

Propriedades

• product name: EJG, 93051803
• fonte biológica: bovine adrenal gland
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Endothelial
• produtos: Factor VIII
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Bovine adrenal medulla endothelial

Descrição de linhagem celular

The bovine cell line was established from collagenase-dissociated, adrenal medulla tissue. The cells display a capillary endothelium morphology with relatively large cell bodies and produce factor VIII:C. Tests for BVDV have shown that the cell line is producing both detectable BVDV antigens and infectious BVDV.

Aplicação

Blood clotting studies

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Passage cells every 5 to 7 days.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.