SBAC

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: bovine adrenal gland (cortex)
• embalagem: tube of 5 μg 91021201-DNA-5UG; pkg of vial of cells 91021201-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Bovine adrenal cortex, normal

Descrição de linhagem celular

Derived from the fasciculata-reticularis zone of the adrenal cortex taken from three normal adult cows. FGF stimulates cell division. Cells are responsive to ACTH and other steroidogenic agents. Finite lifespan; 60-70 population doublings expected. Studies have indicated that this cell line produces both detectable BVDV antigens and infectious BVDV.

Aplicação

Hormone studies

Meio de cultura

Ham′s F12 with Fibroblast Growth Factor (FGF) (crude, 50ng/ml or pure, 500 pg/ml) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.