COLO 800

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93051123, human skin, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human skin
• embalagem: tube of 5 μg 93051123-DNA-5UG; pkg of vial of cells 93051123-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human melanoma

Descrição de linhagem celular

Derived from a subcutaneous nodule of a tumour from a 14-year-old male. Cells display remarkable blebbing.COLO 800 and COLO 794 (Sigma Catalogue number 94072237) were both derived from the same 14 year old male This has been confirmed by STR profiling.

Aplicação

Tumourigenicity studies

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 35°C; 5% CO₂.

Outras notas

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