NB4 1A3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse nerve
• embalagem: tube of 5 μg 89121405-DNA-5UG; pkg of vial of cells 89121405-1VL
• modo de crescimento: Adherent
• cariótipo: Unstable
• morfologia: Neuroblast
• produtos: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse C-1300 Neuroblastoma

Descrição de linhagem celular

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Aplicação

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Meio de cultura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Outras notas

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