SAOS-2 Cell Line human

89050205, human bone, Epithelial-like

• Sinônimo(s): SAOS2 Cells
• Código UNSPSC: 41106514
• fonte biológica: human bone
• modo de crescimento: Adherent
• cariótipo: 2n = 46, (P1) Hyperploid to hypopentaploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: SAOS-2 Cell Line human, 89050205, primary osteogenic sarcoma
• fonte biológica: human bone
• modo de crescimento: Adherent
• cariótipo: 2n = 46, (P1) Hyperploid to hypopentaploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human primary osteogenic sarcoma

Descrição de linhagem celular

Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.

Aplicação

SAOS-2 cell line has been used to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA). It has also been used to study the effect of chromium-enriched diamond-like carbon (DLC) layers on cell adhesion and proliferation.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 18

Meio de cultura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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