SW 48

89012702, human colon, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: SW 48, 89012702
• fonte biológica: human colon
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Epithelial
• produtos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human colon adenocarcinoma

Descrição de linhagem celular

The cells produce a low level of CEA: 0.6ng/1,000,000 cells in 10 days. Cells are tumorigenic in nude mice. Established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35

Aplicação

Tumourigenicity studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,12
D16S539: 11,13
D5S818: 10,14
D7S820: 9,10
THO1: 6,9.3
TPOX: 8
vWA: 18,20

Meio de cultura

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C.

Outras notas

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