WiDr

85111501

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human colon
• modo de crescimento: Adherent
• cariótipo: 2n = 46, hypertriploid
• morfologia: Epithelial
• produtos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human colon adenocarcinoma

Descrição de linhagem celular

Although originally described as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29. HT29 was isolated from a primary colon adenocarcinoma in a 44 year old Caucasian female. The cells produce carcinoembryonic antigen and are tumorigenic in xenogeneic animals. They have been used in studies of anti-tumour agents.

Aplicação

Carcinogenesis and tumourigenicity studies and anti-tumour testing

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19 STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,1

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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