95102433

BEAS-2B Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human lung
• descrição: Human bronchial epithelium, normal
• Formulário: liquid
• embalagem: tube of 5 μg 95102433-DNA-5UG; pkg of vial of cells 95102433-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable

Descrição

Origem de linhagem celular

Human bronchial epithelium, normal

Descrição de linhagem celular

BEAS-2B cells were derived from normal bronchial epithelium obtained from autopsy of non-cancerous individuals. Cells were infected with a replication-defective SV40/adenovirus 12 hybrid and cloned. Squamous differentiation can be observed in response to serum. This ability can be used for screening chemical and biological agents inducing or affecting differentiation and/or carcinogenesis. The cell line has been applied for studies of pneumococcal infection mechanisms. BEAS-2B was described to express keratins and SV40 T antigen. Subculturing the cells before confluency is necessary as confluent cultures rapidly undergo squamous terminal differentiation.

Aplicação

BEAS-2B Cell line has been used to study differentiation of squamous cells and effect of biological and chemical agents on differentiation.

Embalagem

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 13
D16S539: 12
D5S818: 12,13
D7S820: 10,13
THO1: 7,9.3
TPOX: 6,11
vWA: 17,18

Meio de cultura

BEGM, also known as LHC-9 with modification (available from Clonetics Corporation, CC3171 (BEBM) plus additives CC4175 or BEGM Bullet Kit, CC3170); Note: Additives supplied contain gentamycin which has been omitted for culture at ECACC; media is serum-free.

Rotina de subcultura

Passage sub-confluent cultures using 0.25% trypsin or trypsin/EDTA. Incubate at room temperature for 5-10 min until cells detach. Add fresh medium and disperse cells, centrifuge and resuspend pellet in medium. Seed into new flasks at 1500 to 3000 cells per cm². Flasks have to be precoated with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml collagen and 0.001 mg/ml bovine serum albumin dissolved in BEGM. Add mixture at ratio of 0.2 ml per cm² surface area. Incubate at 5% CO₂, 37°C for at least 6 hours at 37oC. Remove excess fluid and allow flasks to dry by incubating at 37oC overnight, leaving the caps loose. Prior to addition of cells wash flask three times with PBS. Note: BEGM medium is almost serum-free, therefore trypsin inhibitor is essential. Add an equal or greater volume of trypsin inhibitor as trypsin used before, centrifuge and reseed cells in collagen coated flasks using culture medium.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line.
Please view the Terms & Conditions of Supply for more information.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable