3T3-F442A
70654, mouse adipose tissue, Fibroblast-like
Sinônimo(s):
3T3F442A Cells, F442A Cells
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About This Item
Código UNSPSC:
41106514
Produtos recomendados
product name
3T3-F442A, 00070654
fonte biológica
mouse adipose tissue
modo de crescimento
Adherent
cariótipo
Not specified
morfologia
Fibroblast-like
produtos
Not specified
receptores
Not specified
técnica(s)
cell culture | mammalian: suitable
Condições de expedição
dry ice
temperatura de armazenamento
−196°C
Origem de linhagem celular
Mouse pre-adipocytes
Descrição de linhagem celular
Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 h.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
Aplicação
Once terminally differentiated the cells can be used as a model for either adipocyte differentiation or mature adipocytes.
Meio de cultura
Rotina de subcultura
Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37°C. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use New
Outras notas
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