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Documentos Principais

C7559

Sigma-Aldrich

Clorofórmio

≥99%, PCR Reagent, contains amylenes as stabilizer

Sinônimo(s):

Tricloreto de metilidino, Triclorometano

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About This Item

Fórmula empírica (Notação de Hill):
CHCl3
Número CAS:
Peso molecular:
119.38
Beilstein:
1731042
Número CE:
Número MDL:
Código UNSPSC:
12191502
ID de substância PubChem:
NACRES:
NA.28

grau

PCR Reagent

Nível de qualidade

densidade de vapor

4.1 (vs air)

pressão de vapor

160 mmHg ( 20 °C)

Ensaio

≥99%

Formulário

liquid

contém

amylenes as stabilizer

embalagem

vial of 1.5 mL

técnica(s)

PCR: suitable

cor

colorless

índice de refração

n20/D 1.445 (lit.)

p.e.

60.5-61.5 °C (lit.)

pf

−63 °C (lit.)

densidade

1.492 g/mL at 25 °C (lit.)

atividade externa

DNase, RNase, none detected

cadeia de caracteres SMILES

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

chave InChI

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Descrição geral

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.

Aplicação

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.

Pictogramas

Skull and crossbonesHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Órgãos-alvo

Central nervous system, Liver,Kidney

Código de classe de armazenamento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves


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Protocolos

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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