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C4482

Sigma-Aldrich

CelLytic™ Y Cell Lysis Reagent

For yeast cells

Sinônimo(s):

Yeast cell lysis reagent

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About This Item

Código UNSPSC:
41116134
NACRES:
NA.56

Nível de qualidade

200

Formulário

solution

Categorias relacionadas

Descrição geral

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Aplicação

CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Características e benefícios

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Informações legais

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H319,H412

Classificações de perigo

Aquatic Chronic 3 - Eye Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
059M4125V
11181021
088M4139V
088M4138V
11180513

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Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

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DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
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Artigos

Yeast Cell Lysis Reagents

Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris and Schizosaccharomyces pombe yeast strains.

Conteúdo relacionado

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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