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Key Documents

C0871

Sigma-Aldrich

Anti-Cullin 3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-CUL3

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About This Item

Número MDL:
MFCD03454920
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen ~80 kDa

reatividade de espécies

rat, mouse, human

concentração

~1.0 mg/mL

técnica(s)

immunoprecipitation (IP): 5-10 μg using lysate of rat brain
indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3

nº de adesão UniProt

Q13618

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... CUL3(8452)
mouse ... Cul3(26554)
rat ... Cul3(301555)

Categorias relacionadas

Descrição geral

Cullin 3 is a member of the cullin family of E3 ubiquitin protein ligases. Cullin 3 forms the BTB-Cul3-Rbx1 (BCR) ubiquitin ligase complex that contains a RING-box protein, Rbx1, and a BTB containing protein. Rbx1 binds to the C-terminus of cullin 3 through its RING-type zinc finger domain to recruit a ubiquitin-conjugating enzyme. The BTB containing protein binds cullin 3 at its N-terminus and functions as a cullin specific adaptor protein.

Aplicação

Anti-Cullin 3 antibody produced in rabbit has been used in
  • western blotting
  • immunoprecipitation
  • immunofluorescence

Ações bioquímicas/fisiológicas

Cullin 3 (Cul3) acts as a key mediator of post-translational protein regulation. It functions as a scaffold protein that assemble multi-subunit ubiquitin ligase complexes which are responsible for the specific recognition and targeting of substrates for ubiquitin-dependent degradation by the 26S proteasome. Cul3 is required for degradation of the microtubule-severing protein MEI-1/katanin during transitioning from meiosis-to-mitosis phase. It forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase and becomes functional upon covalent attachment of the ubiquitin homologue neural-precursor-cell-expressed. BCR ubiquitin ligase down regulated 8 (Nedd8) near the C terminus of Cul3.

forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Missense variants in RHOBTB2 cause a developmental and epileptic encephalopathy in humans, and altered levels cause neurological defects in Drosophila
Straub J, et al.
American Journal of Human Genetics, 102(1), 44-57 (2018)
Lionel Pintard et al.
Nature, 425(6955), 311-316 (2003-09-19)
Many biological processes, such as development and cell cycle progression are tightly controlled by selective ubiquitin-dependent degradation of key substrates. In this pathway, the E3-ligase recognizes the substrate and targets it for degradation by the 26S proteasome. The SCF (Skp1-Cul1-F-box)
Lionel Pintard et al.
The EMBO journal, 23(8), 1681-1687 (2004-04-09)
Cullin-based E3 ligases target substrates for ubiquitin-dependent degradation by the 26S proteasome. The SCF (Skp1-Cul1-F-box) and ECS (ElonginC-Cul2-SOCS box) complexes are so far the best-characterized cullin-based ligases. Their atomic structure has been solved recently, and several substrates have been described
Wananit Wimuttisuk et al.
Molecular biology of the cell, 18(3), 899-909 (2006-12-29)
Cullins are members of a family of scaffold proteins that assemble multisubunit ubiquitin ligase complexes to confer substrate specificity for the ubiquitination pathway. Cullin3 (Cul3) forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of
Rebecca Mathew et al.
The Journal of experimental medicine, 211(6), 1137-1151 (2014-05-28)
Induction of Bcl6 (B cell lymphoma 6) is essential for T follicular helper (Tfh) cell differentiation of antigen-stimulated CD4(+) T cells. Intriguingly, we found that Bcl6 was also highly and transiently expressed during the CD4(+)CD8(+) (double positive [DP]) stage of
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